Ꮭ /tla/ - no, not. Used to negate statements. Eastern equivalent is ᎨᏍᏗ /gesdi/.
-Ꮓ /-hno/ - "declarative clitic" Per Duane King's 1975 dissertation: "In the speech of some Qualla residents, the clitic -hno is frequently used to indicate the beginning of a new sentence or to designate that the sentence is declarative in nature rather than an imperative or interrogative"
(1975:96)". In this function, it is attached to the first word in a sentence. Can be variously translated as "now, well now, so, so then" or sometimes is may not be translated into English.
Note: If -Ꮓ (-hno) is attached to the last word in a series of words, it always means "and". Ex. ᏣᏂ, ᏫᎵ, ᏣᎵᏃ = Johnnie, Willie, and Charlie.
yi- - used with a negation word (Ꮭ in the West, ᎨᏍᏗ in NC) to form a negative sentence. Referred to as the "irrealis prefix", it can be used to form conditional statements and to express doubt or suppositions - 'if' or 'when'. Ex. ᏱᏣᏚᎵᎭ - If you want it. Can also be used in a question to express "would" or "might". Ex. ᏱᎦᏬᏂᎭᏍ ᏣᎳᎩ? Might he speak Cherokee?
ni- - "specified action". Used to indicate a specific action. It is required on a few verbs but doesn't translate.
ga:- - "negative intensifier" when used in conjunction with negator plus /yi-/ irrealis to add extra emphasis. Used with yi- on the Immediate verb stem to express "lack of ability". See Montgomery-Anderson:2015 Cherokee Reference Grammar; p. 299, ex. (44).
h- - "you"; 2nd person singular pronoun, Set A.
-adv:ga - Immediate stem of the verb "to do". See advneha
A fuller translation of this could be "Now you simply cannot do that!"